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Features & Applications

FEATURES

Wide range of accessible vibrations: 740-4000 cm⁻¹
Minor fluorescence interference
High spectral resolution and sensitivity
Sub-wavelength spatial resolution
F-CARS, E-CARS, P-CARS detection geometries
Easy transformable to fluorescence, TPEF and SHG microscopes
Up to 1300 μm excitation for TPEF
Specially designed cost-effective picosecond tunable laser system

APPLICATIONS

Species selective spectroscopy and microscopy
Multimodal nonlinear imaging
Deep tissue in vivo imaging
Long term live cell studies
Non-destructive research for the biological and material sciences
Your application is welcome…

Description

Powerful and versatile tool for vibrational spectroscopy and chemically selective imaging:

Label-free optical imaging
Minimally invasive technique
Non-photobleaching signal
3D sample imaging capability
NIR pump and Stokes wavelengths suitable for deep-tissue imaging
Picosecond pulse duration – good compromise between efficiency and spectral resolution

Coherent anti-Stokes Raman scattering (CARS) spectroscopy primarily was used in chemistry, physics and related fields. It is sensitive to the same vibrational signatures of molecules as seen in Raman spectroscopy, typically the nuclear vibrations of chemical bonds. Unlike Raman spectroscopy, CARS employs multiple photons to address the molecular vibrations, and produces a signal in which the emitted waves are coherent with one another. As a result, CARS is orders of magnitude stronger than spontaneous Raman emission. CARS is a third-order nonlinear optical process involving three laser beams: a pump beam of frequency ω _pump , a Stokes beam of frequency ω _Stokes and a probe beam at frequency ω _probe .

These beams interact with the sample and generate a coherent optical signal at the anti-Stokes frequency ω _CARS = ω _pump – ω _Stokes + ω_probe . The CARS signal ω _CARS is resonantly enhanced when the difference between the pump ω _pump and Stokes ω _Stokesfrequencies matches a vibrational transition ω _vib of the molecule.

Combining of coherent anti-Stokes Raman scattering (CARS) spectroscopy with the microscopy opens up unique method for chemical imaging. CARS microscopy permits vibrational imaging with high-sensitivity, high speed, and three-dimensional nearly diffraction limited spatial resolution.

Coherent anti-Stokes Raman scattering (CARS) spectroscopy principle

Specifications

Model	CARSCOPE-1M-PZ
Optical parameters
Spectral range	740-4000 cm^-1
Pump/probe beams wavelength range	740-990 nm
Stokes wavelength	1064 nm
Spatial resolution	0.5 µm
Scanning system
Scanning method	Sample movement
Scanning device	Three-dimensional piezo stage
Maximum imaging speed ¹⁾	~40 s
Travel range	100x100x20 µm
Resolution	1 nm
Pump laser
Model	PT259-H
Pulse repetition rate ²⁾	1MHz
Tuning range	700-990, 1150-2300 nm
UV extension range (optional)	350-400 nm
Output power ³⁾	25 mW
UV range output power ⁴⁾	1 mW
Linewidth ³⁾	< 8 cm ^-1
Typical pulse duration, ps ^{3) 5)}	~5ps
Typical time bandwidth product	< 0.8
Typical beam diameter ^{3) 6)}	2 mm
Typical beam divergence ^{3) 7)}	< 2 mrad
M²	< 1.6

Measured 200 x 200 pixels.
Inquire for custom pulse repetition rates.
Measured at 800 nm.
Measured at 400 nm.
Pulse duration can vary depending on wavelength and pump energy.
Beam diameter at the FWHM level and can vary depending on the pump pulse energy.
Full angle measured at the FWHM level.

OPTIONS

Fluorescence microscopy (available only with PT259 laser)
Excitation wavelength range	350-400 nm
Detection wavelength range	450-700 nm
TPEF Microscopy
Excitation wavelength range	700-1300 nm
Detection wavelength range	450-900 nm
SHG Microscopy
Excitation wavelength range	800-990, 1064 nm