Product Description

C-Focus™ systems provide an automatic means to eliminate microscope focus drift over long time periods. Unlike autofocus systems which require use of advanced microscopes with internal focus correction or external devices which track video signals or reflected laser beams, the C-Focus™ simply corrects for microscope focus drift by using a high resolution (5nm) sensor system to measure the lens/sample spacing and make the necessary adjustments. The C-Focus™ system has no effect on standard manual focusing and can be retrofit to any microscope. After focus is established, simply pushing the “focus lock” button starts the C-Focus™ operation. Objective lens motions are accomplished with a lens nanopositioner (the Nano-F100S) which is easily connected to the objective lens and microscope with standard threaded adapters. In addition to the unique C-Focus™ operation, lens nanopositioner motion can also be directly commanded by digital (16-bit USB) or analog signals and can be used for high speed, high resolution confocal imaging and other imaging tasks with demanding focus requirements. The C-Focus™ lens positioning system contains the same proprietary, low noise PicoQ^® position sensors used in the Nano-F100S and is capable of sub-nanometer positioning resolution. The 16-bit USB digital computer interface is included with all C-Focus™ controllers.

C-Focus™ Data

Set of Cy3 single molecule imaging
Sample: MutS protein imaged with Cy3 in presence of oxygen scavenging system
Technique: Prism TIRF
Microscope: Olympus IX71
Objective: 60x 1.2 NA Water Immersion, 1.6x magnification
Camera: Hamamatsu EMCCD

Courtesy of:
Biomolecular & Cellular Dynamics Lab
Dept of Physics/IBIO, POSTECH South Korea
http://jblab.postech.ac.kr/

Focus Drift vs. Constant Focus

microscope focal drift correction during comparing with and without focus lock engaged

plot of microscope drift versus focal position during a long duration experiment

Technical Specifications
Range of motion (Nano-F100)	100 μm
Resolution	0.2 nm
Resonant Frequency	500 Hz ±20%
Stiffness	1.0 N/μm
Recommended max. load*	0.5 kg
Body Material	Al and Brass
Controller	C-Focus™
* Larger load requirements should be discussed with our engineering staff.

Additional Information
Nano-F100 Drawing	C-Focus™ System

Product Description

C-Focus™ Data

Focus Drift vs. Constant Focus

Technical Specifications

Additional Information

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